Directed self-assembly of polypeptide-engineered physical microgels for building porous cell-laden hydrogels.
نویسندگان
چکیده
A novel approach to build porous cell-laden hydrogels through the self-assembly of coiled-coil polypeptides on the surface of physical microgels was developed. Both the extracellular microenvironments of pores and physical microgels within assembled constructs could be tailored simultaneously by tuning the polypeptide and morphological features of microgels.
منابع مشابه
Surface-directed assembly of cell-laden microgels.
Cell-laden microscale hydrogels (microgels) can be used as tissue building blocks and assembled to create 3D tissue constructs with well-defined microarchitecture. In this article, we present a bottom-up approach to achieve microgel assembly on a patterned surface. Driven by surface tension, the hydrophilic microgels can be assembled into well-defined shapes on a glass surface patterned with hy...
متن کاملDirected assembly of cell-laden microgels for building porous three-dimensional tissue constructs.
The organization of cells within a well-defined microenvironment is important in generating the resulting tissue function. However, the cellular organization within biodegradable scaffolds often does not resemble those of native tissues. In this study, we present directed assembly of microgels to organize cells for building porous 3D tissue constructs. Cell-laden microgels were generated by mol...
متن کاملDirected assembly of cell-laden microgels for fabrication of 3D tissue constructs.
We present a bottom-up approach to direct the assembly of cell-laden microgels to generate tissue constructs with tunable microarchitecture and complexity. This assembly process is driven by the tendency of multiphase liquid-liquid systems to minimize the surface area and the resulting surface free energy between the phases. We demonstrate that shape-controlled microgels spontaneously assemble ...
متن کاملMethod of Bottom-Up Directed Assembly of Cell-Laden Microgels.
The paper describes a protocol to fabricate cell-laden microgel assemblies with pre-defined micro-architecture and complexity by a bottom-up approach, which can be used for tissue engineering applications. The assembly process was driven by the hydrophobic effect in the water/oil interface. By agitating hydrophilic microgels in hydrophobic medium, the shape-controlled microgel units assemble in...
متن کامل1 Method of Bottom - Up Directed Assembly of Cell - Laden Microgels
9 Abstract—The paper describes a protocol to fabricate cell10 laden microgel assemblies with pre-defined micro-architec11 ture and complexity by a bottom-up approach, which can be 12 used for tissue engineering applications. The assembly 13 process was driven by hydrophobic effect in the water/oil 14 interface. By agitating hydrophilic microgels in hydrophobic 15 medium, the shape-controlled mi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 50 66 شماره
صفحات -
تاریخ انتشار 2014